Comparative Study Between Near Infrared And Raman In The Quantitative Determination Of Pharmaceutical Excipient In Tablets

Abstract

Near infrared (NIR), associated with chemometrics, has been used in deformulation studies, but there is a lack of data regarding the Raman technique when it is applied for these studies. Thus, the objective of the present work was to compare methods of quantitative determination of the excipient hypromellose in desvenlafaxine tablets using NIR and Raman, using chemometrics to treat the results. An experimental design was used to prepare twenty-two calibration samples. The NIR spectra were obtained using a VIAVI MicroNIrTM Spectrometer equipment between 908 and 1676 nm. The Raman spectra were collected using the Raman equipment Cora 501 Anton Paar, between 4,400 and 100,000 nm. The data were treated and compared using the Partial Least Squares (PLS) chemometric method. The raw NIR and Raman calibration spectra showed good resolution after standardization and transformation, which consequently enabled the construction of chemometric models by PLS. The results showed that the models were useful to quantify the excipient hypromellose in desvenlafaxine tablets using both NIR and
Raman. In conclusion: the modeling strategy proved to be viable and the techniques equivalent in the quantification of hypromellose in desvenlafaxine tablets